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How to identify finger prints by using DNA finger printing method.

January 05, 2014  by: Reena  Points: 5   Category: Others  Earning $0.06   Views: 694

DNA finger print is the highly individual specific pattern of variable number of tandem repeats VNTRs in the DNA of a person.

         

DNA finger printing is the method of identification of genetic finger prints.

The procedure involved in DNA finger printing is as follows:-
1)DNA is extracted from the sample and is purified.

2)The purified DNA is treated with restriction endonucleases which cut the DNA at definite points.

3)The fragments of DNA are separated by length by means of a process called agarose gel electrophoresis.

4)The separated fragments of DNA are transferred to a nylon membrane by a process known as blotting.

5)The nylon membrane is immersed in a solution of radioactive probes. The probes hybridize at the VNTR regions known as southern hybridizaton.

6)Finally the VNTR regions present in the DNA fragment are visualized using autoradiography.

Thus the VNTRs of a person are identified i.e, his DNA fingerprint is obtained. The procedure is repeated for DNA sample of another person. The VNTRs of both the persons are compared, i.e., RFLP (Restricted Fragment Length Polymorphism) analysis is done so that their DNA finger prints are distinguished.




Comment(s)
Author: Abhijit Bangal        
Posted Date: 03/11/2014    Points:2    

This is a pretty short article, yet has some contents. When I read the title, I was curious to know what was in. But when I opened it, I saw this miniature article. Doesn''t matter, but there are all the things that were new to me. So, I thought of commenting on it.

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